Now you can control and fine-tune your culture settings to drive cells to differentiation, reprogram them or keep the population as is — reliably and precisely. AVATAR™ gives you advanced control of cell state and population phenotype by letting you tailor gene, protein, or metabolic profiles to derive the target cells you want, when you want them.
Comparison of iPSC differentiation into cardiomyocytes between standard CO2 incubation vs using the AVATAR culture setting of 5% O2 and 2.0 PSI. AVATAR culture conditions (right) resulted in 5X increase in the number of beating cardiomyoctyes after 2 weeks in culture.
Enhanced iPSC maintenance and reprogramming
Pressure affects cell proliferation and cell state, and because AVATAR lets you control it — you now have the ability to create your own cell lines. Tune AVATAR culture settings to promote ‘stemness’ by inducing expression of Nanog, Oct4, Sox2 without the spontaneous, uncontrolled differentiation that comes with standard methods. Cells can also be maintained in a pluripotent state, so you can get to your end goal with fewer steps.
HYPOXIA AND PRESSURE IN COMBINATION CAN PROMOTE MAINTENANCE OF STEMNESS IN iPSCS
Images depict iPSC colonies spontaneously differentiating when supportive fibroblasts are cultured under standard or hypoxic-only culture conditions in minimally supportive media (left and center images). In contrast, application of pressure and low oxygen culture conditions in the AVATAR results in tightly-compacted colonies with high Sox2 expression (purple staining).
Promote targeted differentiation
Using specific culture conditions, AVATAR can consistently control the accuracy and replicability of targeted differentiation of finicky iPSCs from neurons to cardiomyocytes.
ENHANCE iPSC DIFFERENTIATION OF NEURONAL PRECURSOR CELLS BY INDUCING EXPRESSION OF EARLY NEURONAL MARKERS PAX6 AND NESTIN
Neural progenitor cell markers, PAX 6 and NESTIN, are up-regulated in pressure-cultured iPSCs through the AVATAR culture settings at 5% O2 and 2 PSI. (PAX 6 (red), NESTIN (green), DAPI (blue).
qPCR analysis of neural progenitor cells at passage 5 cultured in hypoxic and high-pressure conditions reveal up-regulation of genes involved in neural differentiation (i.e. PAX6) and downregulation in pluripotency maintenance genes (ie NANOG) compared to the same cell lines cultured only in hypoxia.
Promote target protein expression profiles you want or vice versa
Higher induction of PAX6 (red dye), neural progenitor cell (NPC) marker, is accompanied by loss of E-Cadherin (green dye), a negative marker of NPCs indicating iPSCs cultured under hypoxic and pressurized conditions are differentiating into NPCs compared to cells cultured at 18% O2 and at no pressure (left).
AVATAR also lets you differentiate cells faster than standard methods, so you’ll have more to work with earlier on. Cut anywhere from days to months off typical timelines, including the associated reagent costs.
5X INCREASE BEATING CARDIOMYOCYTES AFTER TWO WEEKS COMPARED TO STANDARD METHODS
Enhanced iPSC differentiation into cardiomyocytes. (Left) Day 14, post-differentiation using same culturing reagents with a standard culture method. (Right) Day 14 using the system with 5% O2 and 2 PSI culture conditions result in a 5X increase in the number of beating cardiomyocytes.
Control cell state
AVATAR lets you control cell state and behavior through fine control of the atmospheric conditions experienced by cells. Precise microenvironment control over pressure and oxygen culturing conditions mean you cultivate unique gene, protein, and metabolic profiles of your cells — and improve your cell screening, drug discovery, immunotherapy profiling, and basic biology experiments.
ALTER GENE EXPRESSION PROFILES THROUGH VARYING PRESSURE AND OXYGEN ALONE
Differential gene expression analysis from mRNAseq data of human fibroblasts cultured under varying oxygen and pressure levels show clustering of sample sets based on the pressure value and oxygen level applied. Three different settings of oxygen (1%-16%) and pressure (0-2 PSI) settings were used. Correlation of signature mRNAseq variation to certain settings are highlighted by either pressure or oxygen settings (see blue correlation and orange correlation as accordingly).
AVATAR also lets you hone in on translational differences through protein profiles based on culturing parameters. Changes in culturing conditions don’t just change gene profiles, they can change translational differences too.
TARGETING PROTEIN EXPRESSION: INCREASED mRNA EXPRESSION OF RIBOSOMAL PROTEINS DURING HUMAN FIBROBLASTS CULTURE
Differential gene expression analysis from mRNAseq data of human fibroblasts cultured under varying pressure settings (0, 0.5, 2 PSI from left to right) and oxygen (16%, 5%, 1%) and show increased expression of ribosomal proteins (RPS##) when pressure is applied. Scale bars denote log2fold change (value of +1.0 = +2 fold change).
RAPID CELL EXPANSION
Easily expand primary cells and get up to 8X the cells in the same time as standard methods. Control healthy expansion in patient-derived tumor, immune and stem cell populations by recreating a more relevant microenvironment. Learn more.
UTILIZE KEY CELL TYPES
Work with immune cells, stem cells, tumor cells, organoids and even rare, precious cells you’ve never been able to use before. Only AVATAR lets you regulate and alter both the atmospheric pressure and oxygen concentration to what’s optimal for your cell type. Learn more.