Compatible Cell Types
The AVATAR Cell Control system is tunable to your experiment and supports a range of primary cell types. Our current protocols are optimized for a defined set of immune cell, stem cell, and tumor cell populations. AVATAR is compatible with these cell types:
- Stem Cells
- Immune Cells
- Tumor Cells
From our customers
Ernesto Diaz-Flores Ph.D., Assistant Adjunct Professor
UC San Francisco, Pediatrics/Oncology Department
"While a drug may have great efficacy in peripheral blood where pressure is low, it may not be as efficient in other organs such as the liver due to the increased pressure. To assess efficacy of drugs in conditions mimicking such body conditions may help predict drug responses in vivo and help identify drugs with greater overall efficacy."
Immune Cells: Expanding T-Cells for Immunotherapy Studies
In this novel experiment, target population of non-activated CD8+ T-cells were expanded successfully and more rapidly under higher pressure setting during culture compared to non-pressurized culturing. This shows AVATAR provides enhanced and continued expansion of CD8+ T-cells without the use of exogenous activating compounds.
Using flow cytometry, percent population of non-activated and activated CD8+ T-cells were measured across 3 weeks while being grown in varying pressurized and oxygen conditions in AVATAR.
CARDIOMYOCYTES: TARGETING MATURE CARDIAC PROTEIN PROFILES
In a recent study targeting the expansion of mature cardiomyocytes, cardiomyocytes differentiated in AVATAR under 5% O2 and 2 PSI showed more widespread expression of mature cardiac markers, troponin (green), than cardiomyocytes grown in a standard incubator.
iPSCS: MAINTAINING PLURIPOTENCY IN HUMAN STEM CELLS
AVATAR application of low oxygen and high atmospheric pressure during culture can promote the maintenance of pluripotency in human stem cells. Human stem cells cultured in a standard CO2 incubator (left) exhibit rapid changes in colony morphology and decreased expression pluripotency marker SOX2 in the nuclei (purple staining). In contrast, stem cells cultivated in AVATAR (right) exhibit compact colony morphology and nuclear localization of SOX2, indicative of pluripotency. Learn more.
PROSTATE TUMOR CELLS: EXPANSION OF FRESH PATIENT SAMPLES
In a recent study, scientists from Xcell Biosciences, Knowledge Synthesis, and the Helen Diller Cancer Center at the University of California, San Francisco, studied CTCs from patients with late-stage, castration-resistant prostate cancer to demonstrate the utility of this approach and to characterize the underlying biology of the cells. The cells were cultured in clusters on a collagen-based cell-binding substrate with 2% O2 and a pressure of 2 PSI to mimic the blood environment. The cells, which in traditional incubators typically do not reproduce, were able to survive and propagate in AVATAR.
iPSCs: NEURONAL PRECURSOR CELL DIFFERENTIATION
In collaboration with California Institute of Regenerative Medicine (CIRM), inducing expression of early neuronal markers PAX6 and NESTIN to promote differentiation of iPSCs into Neuronal Precursor Cells (NPCs) was done using AVATAR settings. Induction of PAX6 is accompanied by loss of E-Cadherin (A), indicating iPSCs cultured under pressurized conditions are differentiating into neuronal precursors. Long-term differentiation studies are currently underway for generating mature motor-neurons.
RAPID CELL EXPANSION
Easily expand primary cells and get up to 8X the cells in the same time as standard methods. Control healthy expansion in patient-derived tumor, immune and stem cell populations by recreating a more relevant microenvironment. Learn more.
Easy Cell Control
Drive cells to differentiation or target just the population profiles you need. AVATAR gives you advanced control of cell state by controlling gene, protein, and metabolic profiles. So now you can derive the target cells you want, when you want them. Learn more.