Increase your transfection efficiency up to 13X with high cell viability, even with difficult cells and transfection methods that traditionally produce less than stellar results. Typical transfection methods often weaken your cells. The AVATAR™ Cell Control system is different. It uses high atmospheric pressure enabling efficient delivery of molecular content into a wide range primary cell types with minimal impact on function and viability. Transfection with AVATAR transfection gets rid of all the bottlenecks in developing and deploying your disease modeling and cell-based therapy research. Jumpstart your research with healthy, viable cells post-transfection – and more of them.
Improve transfection efficiency with high viability
Now you don’t have to choose between transfection efficiency or viability – you can have both. Use AVATAR with electroporation, chemical transfection, or lipofection workflows to get higher transfection efficiencies with high cell viability in patient-derived cells. AVATAR lets you squeeze and stress your cells for improved transfection, followed by healthy recovery and cell growth using two key microenvironment conditions – atmospheric pressure and O2 – along with CO2 and temperature.
13X-fold Increase in percent of viable GFP+ multi-cellular clusters post-transfection of PBMCs with CRISPR-based plasmids
PBMCs freshly isolated from healthy donors were enriched for CD8+ cells. CD8+ cells were cultured in a standard incubator or in AVATAR. They were both transfected with CTLA4 CRISPR Knockout and GFP HDR DNA plasmid. Cells expanded with the AVATAR were placed in the AVATAR workflow for transfection while cells cultured in the standard incubator were placed back in conventional culture conditions. Quantification was performed through cell counting across the entire well, and assessed as GFP+ multicellular clusters/total multicellular clusters at 5 days post transfection as well as total cell count at 5 days post transfection. Data represents % GFP+ multicellular clusters, N=4 biological replicates, all transfected at passage 1+ in the exponential growth phase.
See the impact of pressure on donor T-cell transfection
Images taken at day 14 post-transfection of GFP expression in donor T-cells between those transfected through standard culturing conditions and those through high pressure and low oxygen conditions in AVATAR using electroporation transfection methods.
CD8+ cell expansion post transfection
Post-transfection of CRISPR-based plasmids and subsequent expansion of CD8+ cells were cultured in a standard incubator or with AVATAR. Cells expanded with AVATAR not only recovered post-transfection, but expanded in cell count by 2X compared to cells cultured in the standard incubator. Quantification was performed via cell counting across the entire well and assessed as GFP+ multicellular clusters/total multicellular.
High transfection efficiency and high viability? You can have both!
Streamline transfection for reprogramming
AVATAR lets you create any cell type – even novel ones – with efficient transfection that improves reprogramming rates with much higher iPSC colony formation rates. With AVATAR, you can optimize reprogramming efficiency starting with targeted and controlled transfection of reprogramming stemness factors, expansion of iPSCs, and then driving cells to differentiation all in the same instrument.
5X increase in transfection of stemness factors and reprogramming efficiency of iPSCs
- AVATAR Setting #1
- AVATAR Setting #2
- AVATAR Setting #3
Human dermal fibroblasts transfected on AVATAR result in an increase in both transfection and reprogramming efficiency compared to standard methods for iPSC generation depending on which oxygen and pressure settings they were cultured in. With optimized hypoxic and pressurized AVATAR setting #1, a 5-fold increase in iPSC colony formation was seen by day 19 compared to standard methods.
AVATAR transfection workflow
AVATAR streamlines and optimizes the entire transfection process. Precise microenvironments along with custom reagents for before, during and post-transfection support your cells of interest and improve DNA and RNA transfection efficiency.
- Cell preconditioning: Primary cells undergo a ‘preconditioning’ and expansion step prior to transfection using a specific AVATAR setting for the cell type. This moves cells into a growth phase over a 48 to 72-hour period followed by a transfection or reprogramming step.
- Cell recovery: After transfection, cells are cultured using another AVATAR setting for approximately 24 hours to improve cell viability and transfection efficiency.
- Cell expansion: Another AVATAR setting is used for rapid expansion and to enrich for transfected cells.
Rapid Cell Expansion
Easily expand primary cells and get up to 8X the cells in the same time as standard methods. Control healthy expansion in patient-derived tumor, immune and stem cell populations by recreating a more relevant microenvironment. Learn more.
Easy Cell Control
Drive cells to differentiation or target just the population profiles you need. AVATAR gives you advanced control of cell state by controlling gene, protein, and metabolic profiles. So now you can derive the target cells you want, when you want them. Learn more.
Utilize Key Cell Types
Work with immune cells, stem cells, tumor cells, organoids and even rare, precious cells you’ve never been able to use before. Only AVATAR lets you regulate and alter both the atmospheric pressure and oxygen concentration to what’s optimal for your cell type. Learn more.