The AVATAR™ Cell Control System lets you fine-tune oxygen and pressure to cater culture conditions to your cell type of interest. Customizing settings based on tumor type or native microenvironment allows cells to behave as they would in vivo. AVATAR lets you adjust more environmental settings than traditional incubators. So you can now study cells under a range of conditions and find out which combination of factors is ideal for each type of cell. Because AVATAR mimics a more relevant human microenvironment, cells stay happy and healthy too.
This gallery is a collection of images, taken using immunofluorescence microscopy, of various tumor samples that were successfully grown using the AVATAR System. Each tumor type was grown using a combination of low oxygen and high pressure to mimic the specific tumor microenvironment, facilitating better tumor expansion
Patient Tumor Samples Successfully Grown in the AVATAR System
- Lung Cancer – NSCLC
- Lung Cancer – Small-cell Carcinoma
- Breast Cancer – Triple-negative
- Breast Cancer – Calcified (Hard Tissue)
- Breast Cancer – Liquefied (Soft Tissue)
- Ovarian Cancer – Ascites
- Colorectal Cancer – Adenocarcinoma
- Liver Cancer – Hepatocellular Carcinoma
- Pancreatic Cancer – PDAC
- Prostate Cancer – mCRPC
- Kidney Cancer – Renal Cell Carcinoma (RCC)
- Esophagal Cancer – Squamous-cell Carcinoma
- Circulating Tumor Cells (CTCs)
- Multiple Myeloma Leukemia (MML)
- Chronic Myeloid Leukemia (CML)
- Acute Myeloid Leukemia (AML)
- Mixed Lineage Leukemia (MLL)
- Low Grade Glioma
Enabling 3-D Organoid Culture
Primary patient-derived pancreatic ductal adenocarcinoma sample was processed and plated on collagen-based hyrogel-coated plates. Samples were fed every 4 days and cultured for 8+ weeks in a low oxygen (1% O2) and high hydrostatic pressure (2 PSI) environment using the AVATAR System. Spheroids were stained using DAPI (blue), CK7/CK19 (green), and EpCAM (red).
Expand & Maintain Identity of Patient-derived Tumor Samples
Lymph node FNA (Fine Needle Aspirate) of a patient PDAC sample was obtained on the day of surgery and processed within 3 hours upon receipt from O.R.. Samples were seeded on collagen-based hyrogel-coated plates and grown in the AVATAR System under low oxygen and high hydrostatic pressure (1% O2 + 2 PSI). Cells were fed with chemically defined culture media every 4 days and grown in culture for 8+ weeks. Cells were imaged at 3 time points (2+ weeks, 4+ weeks, and 8+ weeks) and imaged using brightfield and immunofluorescence imaging. Samples were stained using DAPI (blue), CK7/CK19 (green), and EpCAM (red). Cells were propagated from less than a few hundred viable cells and were expanded to ~2×105 cells in 8 weeks. Cells were passaged and further expanded to ~2×106 for additional 8 weeks (not shown). Additionally, the pancreatic cancer cells (red arrows) are selected for under long-term culture in the tumor microenvironment, eliminating all unneeded stromal fibroblasts (white arrows) from their initial co-culture seeding.
Xcell Bio’s Complete Tumor Expansion Workflow
Our Dissoci8 Kit lets you dissociate patient-derived samples from tumors, fine-needle aspirates, and punch biopsies, then enrich them with our proprietary Xcellr8 media formulation for greater and more consistent expansion.
- Primary sample prep: Our Dissoc8 Kit is used for tumor dissociation and target cell isolation.
- Cell expansion: Tumor microenvironments are replicated with a combination of our Xcellr8 Kit, XCM plates, and AVATAR systems enabling hypoxia and pressure control for optimal tumor cell expansion.